Journal: Clinical Epigenetics
Article Title: Improved results of LINE-1 methylation analysis in formalin-fixed, paraffin-embedded tissues with the application of a heating step during the DNA extraction process
doi: 10.1186/s13148-016-0308-0
Figure Lengend Snippet: Mean methylation levels of the four LINE-1 CpG sites for xenograft tissue samples ( n = 10) with five different durations of formalin fixation. DNA samples were heat-untreated ( a ) and heat-untreated ( b ). Both the paired Student’s t test and the paired Wilcoxon signed-rank test were performed to compare the mean methylation values of the four CpG sites between paired fresh-frozen and formalin-fixed, paraffin-embedded tissue samples. P values in the bar graph represent the values of both the parametric and the non-parametric tests
Article Snippet: More recently, however, instead of using the MethyLight-based LINE-1 methylation assay, a pyrosequencing-based LINE-1 methylation assay has become widely used because the pyrosequencing-based assay has been validated for its precision and reliability in formalin-fixed, paraffin-embedded (FFPE) tissue samples by Irahara et al. [ ].
Techniques: Methylation, Formalin-fixed Paraffin-Embedded